Optical Chemical Sensor Based on Fast-Protein Liquid Chromatography for Regular Peritoneal Protein Loss Assessment in End-Stage Renal Disease Patients on Continuous Ambulatory Peritoneal Dialysis

Point-of-care testing (POCT) devices are becoming increasingly popular in the medical community as an alternative to conventional laboratory testing, especially for home treatments or other forms of outpatient care. Multiple-use chemical sensors with minimal requirements disposables among most practical and cost-effective POC diagnostic instruments, managing chronic conditions. An affordable, simple, easy-to-use optical sensor based on fast protein liquid chromatography direct UV absorption detection was developed rapid determination total concentration effluent peritoneal dialysate assessment losses end-stage renal disease (ESRD) patients constant ambulatory dialysis (CAPD). The employs non-disposable PD-10 desalting columns separation molecules different molecular weights a deep LED (maximum at 285 nm) light source detection. analytic procedure is relatively takes 10–15 min, potentially can be performed by themselves nursing staff without training. Preliminary clinical trials group 23 CAPD revealed good concordance between concentrations samples measured automated biochemical analyzer; mean relative error about 10%, which comparable routine methods.